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Endothelial Cell Surface Expressed Chemotaxis and Apoptosis Regulator (ECSCR) Regulates Lipolysis in White Adipocytes via the PTEN/AKT Signaling Pathway. PLoS One 2015;10(12):e0144185

Date

12/23/2015

Pubmed ID

26692198

Pubmed Central ID

PMC4686900

DOI

10.1371/journal.pone.0144185

Scopus ID

2-s2.0-84956927768   7 Citations

Abstract

Elevated plasma triglycerides are associated with increased susceptibility to heart disease and stroke, but the mechanisms behind this relationship are unclear. A clearer understanding of gene products which influence plasma triglycerides might help identify new therapeutic targets for these diseases. The Endothelial Cell Surface expressed Chemotaxis and apoptosis Regulator (ECSCR) was initially studied as an endothelial cell marker, but has recently been identified in white adipocytes, the primary storage cell type for triglycerides. Here we confirm ECSCR expression in white adipocytes and show that Ecscr knockout mice show elevated fasting plasma triglycerides. At a cellular level, cultured 3T3-L1 adipocytes silenced for Ecscr show a blunted Akt phosphorylation response. Additionally we show that the phosphatase and tensin homology containing (PTEN) lipid phosphatase association with ECSCR is increased by insulin stimulation. These data suggest a scenario by which ECSCR contributes to control of white adipocyte lipolysis. In this scenario, white adipocytes lacking Ecscr display elevated PTEN activity, thereby reducing AKT activation and impairing insulin-mediated suppression of lipolysis. Collectively, these results suggest that ECSCR plays a critical function in regulating lipolysis in white adipose tissue.

Author List

Kilari S, Cossette S, Pooya S, Bordas M, Huang YW, Ramchandran R, Wilkinson GA

Authors

Yi-Wen Huang PhD Assistant Professor in the Obstetrics and Gynecology department at Medical College of Wisconsin
Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

3T3-L1 Cells
Adipocytes, White
Animals
Apoptosis Regulatory Proteins
Lipolysis
Mice
Mice, Knockout
PTEN Phosphohydrolase
Proto-Oncogene Proteins c-akt
Signal Transduction
Triglycerides
jenkins-FCD Prod-400 0f9a74600e4e79798f8fa6f545ea115f3dd948b2