Characterization of CC-531 as a Rat Model of Colorectal Liver Metastases. PLoS One 2016;11(5):e0155334
Date
05/14/2016Pubmed ID
27171151Pubmed Central ID
PMC4865145DOI
10.1371/journal.pone.0155334Scopus ID
2-s2.0-84969528718 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
PURPOSE: Surgical resection of colorectal liver metastases is not achievable in more than 70% of the cases. Although the liver directed therapies have become a part of the stand of care, lack of a preclinical model impedes the assessment of toxicity and therapeutic benefits attributed several candidate drugs or treatment regimens that can be designed. In the present study we aim develop and characterize a rat colorectal liver metastasis model.
MATERIALS AND METHODS: Growth characteristics of CC-531 cells were determined in vitro followed by subcapsular liver implantation in syngeneic WAG/Rij rats. Tumor growth progression was followed over 3 weeks by ultrasound (US) and magnetic resonance imaging (MRI). Growth characteristics were also assessed by histopathology and immunohistochemistry in harvested tumor tissues.
RESULTS: The doubling time of CC-531 cells was found be under 24hrs and all the implanted rats grew tumors. US imaging showed hypoechoic masses and MRI showed contrast enhancement representing complex tumor microenvironments. Hematoxylin and Eosin staining confirmed tumor growth and uniform CD31 staining in tumor confirmed even vessel density.
CONCLUSION: CC-531 can be used as a metastatic rat tumor colorectal liver metastases model with well-defined characteristics that can be readily followed by imaging whilst having a therapeutic window for interventions.
Author List
White SB, Procissi D, Chen J, Gogineni VR, Tyler P, Yang Y, Omary RA, Larson ACAuthor
Sarah B. White MD, MS, FSIR, FCIRSE Associate Dean, Vice Chair, Professor in the Radiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Line, Tumor
Cell Proliferation
Colorectal Neoplasms
Disease Models, Animal
Electroporation
Immunohistochemistry
Liver Neoplasms
Magnetic Resonance Imaging
Rats
