Proteomic analysis of human mesenteric lymph. Shock 2011 Apr;35(4):331-8
Date
12/31/2010Pubmed ID
21192285Pubmed Central ID
PMC3059368DOI
10.1097/SHK.0b013e318206f654Scopus ID
2-s2.0-79961090433 (requires institutional sign-in at Scopus site) 42 CitationsAbstract
Extensive animal work has established mesenteric lymph as the mechanistic link between gut ischemia/reperfusion and distant organ injury. Our trauma and transplant services provide a unique opportunity to assess the relevance of our animal data to human mesenteric lymph under conditions that simulate those used in the laboratory. Mesenteric lymph was collected from 11 patients with lymphatic injuries, during semielective spine reconstruction or immediately before organ donation. The lymph was tested for its ability to activate human neutrophils in vitro and was analyzed by label-free proteomic analysis. Human mesenteric lymph primed human polymorphonuclear neutrophils in a pattern similar to that observed in previous rodent, swine, and primate studies. A total of 477 proteins were identified from the 11 subjects' lymph samples with greater than 99% confidence. In addition to classic serum proteins, markers of hemolysis, extracellular matrix components, and general tissue damage were identified. Both tissue injury and shock correlate strongly with production of bioactive lymph. Products of red blood cell hemolysis correlate strongly with human lymph bioactivity, and immunoglobulins have a negative correlation with the proinflammatory lymph. These human data corroborate the current body of research implicating postshock mesenteric lymph in the development of systemic inflammation and multiple organ failure. Further studies will be required to determine if the proteins identified participate in the pathogenesis of multiple organ failure and if they can be used as diagnostic markers.
Author List
Dzieciatkowska M, Wohlauer MV, Moore EE, Damle S, Peltz E, Campsen J, Kelher M, Silliman C, Banerjee A, Hansen KCMESH terms used to index this publication - Major topics in bold
Acute-Phase ProteinsCarrier Proteins
Cells, Cultured
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Hemolysis
Humans
Lymph Nodes
Membrane Glycoproteins
Mesentery
Mitochondrial Proteins
Neutrophils
Oxidative Stress
Proteomics
Superoxides
Tandem Mass Spectrometry