A single center's approach to discriminating donor versus host origin of renal neoplasia in the allograft kidney. Ann Diagn Pathol 2016 Aug;23:32-4
Date
07/13/2016Pubmed ID
27402221DOI
10.1016/j.anndiagpath.2016.05.004Scopus ID
2-s2.0-84974715392 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
Renal cell carcinoma (RCC) in the allograft of kidney transplant recipient (KTR) patients is rare and may represent a de novo process arising from the transplanted organ or metastasis from a clinically undetectable host primary. Determination of host versus donor origin is important for staging and management. We report our experience utilizing Penta-C (PC) and Penta-D (PD) short-tandem repeat (STR) microsatellite analysis to discriminate between host and donor origin of RCC identified in renal allografts. We identified 5 KTR patients with RCC in the allograft kidney. The PC and PD microsatellite analysis was applied to tumor, host, and donor formalin-fixed, paraffin-embedded tissue sections and/or fresh blood leukocytes to identify the origin of the neoplastic cells. The PC and PD microsatellite alleles were robustly amplified in all samples. Each case showed one or more informative alleles indicating that the neoplastic cells originate from donor tissue. Allele frequency data indicate that by using both PC and PD markers, we will be able to discriminate between host and donor cell of origin in over 99% of cases. The PC and PD microsatellite analysis is a convenient, robust, and efficient strategy to determine donor versus host origin or RCC in transplant kidney specimens.
Author List
Robin AJ, Cohen EP, Chongkrairatanakul T, Saad E, Mackinnon ACMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Carcinoma, Renal Cell
Female
Humans
Kidney Neoplasms
Kidney Transplantation
Male
Microsatellite Repeats
Middle Aged
Tissue Donors
Transplantation, Homologous
Young Adult