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Live Attenuated Borrelia burgdorferi Targeted Mutants in an Infectious Strain Background Protect Mice from Challenge Infection. Clin Vaccine Immunol 2016 08;23(8):725-31 PMID: 27335385 PMCID: PMC4979176

Pubmed ID

27335385

DOI

10.1128/CVI.00302-16

Abstract

Borrelia burgdorferi, B. garinii, and B. afzelii are all agents of Lyme disease in different geographic locations. If left untreated, Lyme disease can cause significant and long-term morbidity, which may continue after appropriate antibiotic therapy has been administered and live bacteria are no longer detectable. The increasing incidence and geographic spread of Lyme disease are renewing interest in the vaccination of at-risk populations. We took the approach of vaccinating mice with two targeted mutant strains of B. burgdorferi that, unlike the parental strain, are avirulent in mice. Mice vaccinated with both strains were protected against a challenge with the parental strain and a heterologous B. burgdorferi strain by either needle inoculation or tick bite. In ticks, the homologous strain was eliminated but the heterologous strain was not, suggesting that the vaccines generated a response to antigens that are produced by the bacteria both early in mammalian infection and in the tick. Partial protection against B. garinii infection was also conferred. Protection was antibody mediated, and reactivity to a variety of proteins was observed. These experiments suggest that live attenuated B. burgdorferi strains may be informative regarding the identification of protective antigens produced by the bacteria and recognized by the mouse immune system in vivo Further work may illuminate new candidates that are effective and safe for the development of Lyme disease vaccines.

Author List

Hahn BL, Padmore LJ, Ristow LC, Curtis MW, Coburn J

Author

Jenifer Coburn PhD Professor in the Medicine department at Medical College of Wisconsin




Scopus

2-s2.0-84982994177   1 Citations

MESH terms used to index this publication - Major topics in bold

Animal Structures
Animals
Antibodies, Bacterial
Borrelia burgdorferi
Disease Models, Animal
Female
Lyme Disease
Lyme Disease Vaccines
Mice, Inbred C3H
Ticks
Vaccines, Attenuated
Virulence
jenkins-FCD Prod-332 f92a19b0ec5e8e1eff783fac390ec127e367c2b5