Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein implicated in vesicle recycling. J Biol Chem 1999 Sep 03;274(36):25517-24
Date
08/28/1999Pubmed ID
10464283DOI
10.1074/jbc.274.36.25517Scopus ID
2-s2.0-0033520460 (requires institutional sign-in at Scopus site) 83 CitationsAbstract
Rab11 small G protein has been implicated in vesicle recycling, but its upstream regulators or downstream targets have not yet been identified. We isolated here a downstream target of Rab11, named rabphilin-11, from bovine brain. Moreover, we isolated from a rat brain cDNA library its cDNA, which encoded a protein with a M(r) of 100,946 and 908 amino acids (aa). Rabphilin-11 bound GTP-Rab11 more preferentially than GDP-Rab11 at the N-terminal region and was specific for Rab11 and inactive for other Rab and Rho small G proteins. Both GTP-Rab11 and rabphilin-11 were colocalized at perinuclear regions, presumably the Golgi complex and recycling endosomes, in Madin-Darby canine kidney cells. In HeLa cells cultured on fibronectin, both the proteins were localized not only at perinuclear regions but also along microtubules, which were oriented toward membrane lamellipodia. Treatment of HeLa cells with nocodazole caused disruption of microtubules and dispersion of GTP-Rab11 and rabphilin-11. Overexpression of the C-terminal fragment of rabphilin-11 (aa 607-730), lacking the GTP-Rab11 binding domain, in HeLa cells reduced accumulation of transferrin at perinuclear regions and cell migration. Rabphilin-11 turned out to be a rat counterpart of recently reported bovine Rab11BP. These results indicate that rabphilin-11 is a downstream target of Rab11 which is involved in vesicle recycling.
Author List
Mammoto A, Ohtsuka T, Hotta I, Sasaki T, Takai YAuthor
Akiko Mammoto MD, PhD Associate Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Antineoplastic Agents
Biological Transport
Cattle
Cytoplasmic Granules
Dogs
GTP-Binding Proteins
HeLa Cells
Humans
Microtubules
Molecular Sequence Data
Nocodazole
Rats
rab GTP-Binding Proteins
