Inhibition of mouse placental lactogen-II release from placental cells by interleukin-1 after mid-pregnancy. J Endocrinol 1995 Dec;147(3):423-9
Date
12/01/1995Pubmed ID
8543912DOI
10.1677/joe.0.1470423Scopus ID
2-s2.0-0028834308 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
The effects of interleukin (IL)-1 and granulocyte-macrophage colony stimulating factor (GM-CSF), which are present in the mouse placenta, on the secretion of mouse placental lactogen (mPL)-1 and mPL-II by placental cells were tested in vitro. IL-1 alpha and IL-1 beta, 2.5 nmol/l each, significantly inhibited mPL-II secretion by cells from days 9 and 12 of pregnancy, but did not affect mPL-II secretion by cells from day 7 of pregnancy or mPL-I secretion by cells from days 7, 9 or 12 of pregnancy. GM-CSF had no effect on mPL-I and mPL-II secretion by cells from days 7, 9 or 12 of pregnancy. The inhibitory effects of IL-1 alpha and IL-1 beta on mPL-II secretion were completely eliminated by the addition of antibodies to IL-1 alpha and IL-1 beta respectively. Western blot analysis for mPL-II indicated that IL-1 alpha significantly reduced the intensity of the mPL-II band. Steady-state levels of mPL-II mRNA, assessed by Northern blot analysis, were reduced by incubation of placental cells from day 12 of pregnancy with 2.5 nmol/l IL-1 alpha for 5 days. Co-incubation of 0.25 pmol/l IL-1 alpha, 25 pmol/l IL-6, and 25 pmol/l tumor necrosis factor-alpha, each of which did not significantly inhibit mPL-II secretion by itself, together inhibited mPL-II secretion. These results suggest that IL-1, but not GM-CSF, is a potent inhibitor of mPL-II secretion after mid-pregnancy, and that the combined action of cytokines can inhibit mPL-II secretion.
Author List
Yamaguchi M, Sakata M, Ogura K, Adachi K, Mammoto A, Miyake AAuthor
Akiko Mammoto MD, PhD Associate Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlotting, Northern
Blotting, Western
Cells, Cultured
Female
Granulocyte Colony-Stimulating Factor
Interleukin-1
Mice
Mice, Inbred ICR
Placenta
Placental Lactogen
Pregnancy
Pregnancy, Animal
RNA, Messenger
Radioimmunoassay