Regulation of mouse soluble interleukin-6 receptor secretion by decidua. Biochem Biophys Res Commun 1995 Jun 26;211(3):1077-82
Date
06/26/1995Pubmed ID
7598695DOI
10.1006/bbrc.1995.1921Scopus ID
2-s2.0-0029010178 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Regulation of msIL-6R secretion by mIL-6 and 8-bromo cAMP was examined using primarily cell culture of mouse decidua. Mouse decidua on day 11 of pregnancy was digested by collagenase and decidual cells were cultured for up to 3 days. Addition of mIL-6 and 8-bromo cAMP resulted in significant inhibition of msIL-6R secretion from cultured mouse decidual cells by the 2nd day in culture. The effects were dose-dependent and the lowest concentrations of mIL-6 and 8-bromo cAMP which cause significant inhibition of msIL-6R secretion were 250 pM and 50 microM, respectively. Northern blot analysis indicated that treatment of decidual cells with 8-bromo cAMP significantly decreased the steady-state level of mIL-6R mRNA. However, treatment of decidual cells with mIL-6 did not affect the steady-state level of mIL-6R mRNA. These results suggest that mIL-6 decreases msIL-6R secretion without changing of the steady-state level of mIL-6R mRNA, and that cAMP is one of the second messengers in mouse decidual cells involved in this down regulation of msIL-6R secretion.
Author List
Yamaguchi M, Mammoto A, Taga T, Kishimoto T, Miyake AAuthor
Akiko Mammoto MD, PhD Associate Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
8-Bromo Cyclic Adenosine MonophosphateAnimals
Cells, Cultured
Decidua
Dose-Response Relationship, Drug
Female
Gene Expression Regulation
Interleukin-6
Mice
Mice, Inbred ICR
Pregnancy
RNA, Messenger
Receptors, Interleukin
Receptors, Interleukin-6
