Immune destruction of human platelets in the NOD/scid mouse. Transfusion 2016 Oct;56(10):2648-2649
Date
10/16/2016Pubmed ID
27739154Pubmed Central ID
PMC5111357DOI
10.1111/trf.13746Scopus ID
2-s2.0-84991672237 (requires institutional sign-in at Scopus site)Abstract
Recently Fuhrmann et al published in Transfusion an article on optimization of the NOD/scid mouse model for studying survival kinetics and immune clearance of human platelets in vivo (Transfusion, Apr 18, 2016). An additional unresolved issue concerning this model is whether cross-species incompatibility between human IgG Fc and mouse Fc receptors (FcR) adversely affects antibody-induced platelet clearance. If so, it might be necessary to use mice transgenic for human FcR to optimize the model for study of immune destruction of human blood cells. We used the IgG1 mouse monoclonal 7E3, specific for human GPIIb/IIIa, a humanized variant of 7E3 (c7E3) and c7E3 Fab fragments to show that the inter-species FcR difference impairs clearance of human platelets sensitized with Ig1 antibodies only slightly and cite in vitro studies suggesting that the same is likely to be true of IgG3 antibodies, the second most common pathogenic antibody isotype. Findings made validate use of the NOD/scid mouse model for the study of antibody-mediated clearance of human blood cells.
Author List
Bougie DW, Nayak D, Aster RHMESH terms used to index this publication - Major topics in bold
AnimalsBlood Platelets
Humans
Mice
Mice, Inbred NOD
Mice, SCID
Severe Combined Immunodeficiency
Transplantation, Heterologous
