Multicenter Evaluation of the Portrait Staph ID/R Blood Culture Panel for Rapid Identification of Staphylococci and Detection of the mecA Gene. J Clin Microbiol 2017 Apr;55(4):1140-1146
Date
01/27/2017Pubmed ID
28122871Pubmed Central ID
PMC5377841DOI
10.1128/JCM.02348-16Scopus ID
2-s2.0-85016173124 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
Bloodstream infections are a leading cause of morbidity and mortality in the United States and are associated with increased health care costs. We evaluated the Portrait Staph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) for the rapid and simultaneous identification (ID) of Staphylococcus aureus, Staphylococcus lugdunensis, and Staphylococcus species to the genus level and the detection of the mecA gene directly from a positive blood culture bottle. A total of 765 Bactec bottles demonstrating Gram-positive cocci in singles or clusters were tested during the prospective trial at 3 clinical sites. The Portrait Staph ID/R BCP results were compared with results from conventional biochemical and cefoxitin disk methods performed at an independent laboratory. Discordant ID and mecA results were resolved by rpoB gene sequencing and mecA gene sequencing, respectively. A total of 658 Staphylococcus species isolates (S. aureus, 211 isolates; S. lugdunensis, 3 isolates; and Staphylococcus spp., 444 isolates) were recovered from monomicrobial and 33 polymicrobial blood cultures. After discrepant analysis, the overall ratios of Portrait Staph ID/R BCP positive percent agreement and negative percent agreement were 99.4%/99.9% for Staphylococcus ID and 99.7%/99.2% for mecA detection.
Author List
Denys GA, Collazo-Velez V, Young S, Daly JA, Couturier MR, Faron ML, Buchan BW, Ledeboer NAuthors
Blake W. Buchan PhD Professor in the Pathology department at Medical College of WisconsinNathan A. Ledeboer PhD Vice Chair, Professor in the Pathology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Blood CultureGenes, Bacterial
Humans
Methicillin Resistance
Multiplex Polymerase Chain Reaction
Prospective Studies
Staphylococcal Infections
Staphylococcus
Time Factors
United States