Anti-idiotype-induced, lipopolysaccharide-specific antibody response to Pseudomonas aeruginosa. II. Isotype and functional activity of the anti-idiotype-induced antibodies. J Immunol 1991 Jan 01;146(1):188-93
Date
01/01/1991Pubmed ID
1898598Scopus ID
2-s2.0-0026068955 (requires institutional sign-in at Scopus site) 37 CitationsAbstract
We recently developed a murine anti-idiotypic mAb that functioned as a molecular mimic of the O-specific polysaccharide side chain (Ps) of Pseudomonas aeruginosa LPS in vitro, and which induced Ps-specific antibodies in syngeneic BALB/c ByJ mice. In the current studies, we demonstrate that these anti-Id-induced, Ps-specific antibodies fix complement to the bacterial cell surface, and protect neutropenic mice from fatal P. aeruginosa sepsis. The isotypic distribution of the anti-Id-induced antibodies, however, resembles the restricted pattern (IgM and IgG3) seen after administration of purified Ps to mice. The immunogenicity and number of isotypes of Ps-specific antibody produced could be enhanced by conjugating the anti-Id to keyhole limpet hemocyanin. Finally, the anti-Id administered before immunization with purified Ps, primed BALB/c ByJ mice for production of other Ps-specific antibody isotypes (IgG1). These studies show that this anti-Id induces functional anti-Ps antibodies in syngeneic mice, and when used in conjugate form or as a priming agent before Ps immunization, yields an antibody response consistent with "T cell dependence." These immunization strategies may be useful for the induction of polysaccharide-specific antibodies in man.
Author List
Schreiber JR, Nixon KL, Tosi MF, Pier GB, Patawaran MBMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies, Anti-Idiotypic
Antibodies, Bacterial
Complement Fixation Tests
Hemocyanins
Immunization, Passive
Immunoglobulin Idiotypes
Immunoglobulin Isotypes
Lipopolysaccharides
Mice
Mice, Inbred BALB C
Molecular Weight
Pseudomonas Infections
Pseudomonas aeruginosa