Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer. Sci Rep 2017 Jun 23;7(1):4150
Date
06/25/2017Pubmed ID
28646161Pubmed Central ID
PMC5482839DOI
10.1038/s41598-017-04386-1Scopus ID
2-s2.0-85021303662 (requires institutional sign-in at Scopus site) 148 CitationsAbstract
Circulating exosome holds great potentials as biomarker for diagnosis and prognosis of human cancers. Previously, we have applied small RNA sequencing to identify aberrantly expressed exosomal miRNAs as candidates for diagnostic markers in colon cancer patients. In this validation cohort, plasma derived exosomal miRNA was isolated from 50 early-stage colon cancer patients and 50 matched healthy volunteers. Real-time qRT-PCR revealed that miR-125a-3p, miR-320c were significantly up-regulated in plasma exosomes of the patients with early stage colon cancer. ROC curve showed that miR-125a-3p abundant level may predict colon cancer with an area of under the curve (AUC) of 68.5%, in comparison to that of CEA at 83.6%. Combination of miR-125a-3P and CEA improved the AUC to 85.5%. In addition, plasma exosome level of miR-125a-3p and miR-320c showed significant correlation with nerve infiltration (P < 0.01), but not with tumor size, infiltration depth, and differentiation degree (P > 0.05). On the contrary, plasma CEA level is correlated with tumor size, infiltration depth, and differentiation degree (P < 0.05, r = 0.3009-0.7270), but not with nerve infiltration (P = 0.744). In conclusion, this follow-up study demonstrated circulating plasma exosomal miR-125a-3p is readily accessible as diagnosis biomarker for early-stage colon cancer. When combined with conventional diagnostic markers, miR-125a-3p can improve the diagnostic power.
Author List
Wang J, Yan F, Zhao Q, Zhan F, Wang R, Wang L, Zhang Y, Huang XMESH terms used to index this publication - Major topics in bold
AgedBiomarkers, Tumor
Carcinoembryonic Antigen
Case-Control Studies
Circulating MicroRNA
Colonic Neoplasms
Exosomes
Female
Gene Expression Regulation, Neoplastic
Humans
Male
MicroRNAs
Middle Aged
Neoplasm Staging
ROC Curve