The Xenopus oocyte: a single-cell model for studying Ca2+ signaling. Cold Spring Harb Protoc 2013 Mar 01;2013(3)
Date
03/05/2013Pubmed ID
23457336Pubmed Central ID
PMC4017334DOI
10.1101/pdb.top066308Scopus ID
2-s2.0-84874704380 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
In the four decades since the Xenopus oocyte was first demonstrated to have the capacity to translate exogenous mRNAs, this system has been exploited for many different experimental purposes. Typically, the oocyte is used either as a "biological test tube" for heterologous expression of proteins without any particular cell biological insight or, alternatively, it is used for applications where cell biology is paramount, such as investigations of the cellular adaptations that power early development. In this article, we discuss the utility of the Xenopus oocyte for studying Ca(2+) signaling in both these contexts.
Author List
Lin-Moshier Y, Marchant JSAuthor
Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCalcium
Cytological Techniques
Oocytes
Signal Transduction
Xenopus
