Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Intramucosal distribution of WNT signaling components in human esophagus. J Clin Gastroenterol 2009 Apr;43(4):327-37 PMID: 19309775 PMCID: PMC4811354

Abstract

BACKGROUND: The molecular mechanisms governing the biology and pathobiology of esophageal squamous mucosa in health and disease are not completely understood. Earlier genome-wide expression study of normal-looking esophageal squamous mucosa has shown differential expression of the Wingless-type MMTV integration site family (Wnt) modulators Dickkopf (Dkk) homologs among healthy individuals and patients with reflux esophagitis and Barrett metaplasia suggesting that the Wnt pathway may be involved in esophageal mucosal biology.

STUDY: Seven full-thickness human donor esophagi were cryosectioned for immunohistochemical analysis, and lamina propria (LP), basal (BC), intermediate (IC), and superficial (SC) cells were also dissected by laser-capture microdissection for real-time polymerase chain reaction.

RESULTS: Wnt1, 2b, and 3a were expressed primarily in BC, Wnt3, and 5b in LP, and Wnt5a in IC. Frizzled 1, low-density lipoprotein receptor-related protein 6, secreted frizzled-related protein 1, T-cell-specific transcription factor 3, and dishevelled 3 were expressed highest in LP decreasing precipitously medially toward SC. Dkk1 predominantly expressed in SC was more than 100-folds greater than other layers (P<0.001). Dkk4 was expressed primarily in SC but Dkk3 was opposite with greatest expression in LP. Immunohistochemical analysis showed Wnt1 and 3a in BC, Wnt5a in IC and SC, Dkk1 predominantly in SC, Dkk4 in SC and IC, and Dkk3 and SFRP1 in LP and BC

Author List

Ali I, Rafiee P, Zheng Y, Johnson C, Banerjee B, Haasler G, Jacob H, Shaker R

Authors

Banani Banerjee PhD Associate Professor in the Medicine department at Medical College of Wisconsin
Reza Shaker MD Sr Associate Dean, Ctr Director, Chief, Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Epithelium
Esophagus
Frizzled Receptors
Gene Expression Profiling
Gene Expression Regulation
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins
Intracellular Signaling Peptides and Proteins
Lasers
Ligands
Membrane Proteins
Microdissection
Mucous Membrane
Polymerase Chain Reaction
Signal Transduction
Transcription Factors
Wnt Proteins



View this publication's entry at the Pubmed website PMID: 19309775
jenkins-FCD Prod-141 f776ee82366dc5656a368d51cce32734d2f9b9a0