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The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals. J Cachexia Sarcopenia Muscle 2013 Sep;4(3):239-43 PMID: 23529675 PMCID: PMC3774913

Abstract

BACKGROUND: Doxorubicin treatment is known to cause muscular weakness. However, the cellular mechanisms have not been elucidated. We aimed to determine the effects of acute doxorubicin treatment on proteome lysine acetylation status, an indication of the apoptotic and inflammatory environment, and the expression and activation of various apical caspases involved in the initiation of apoptosis.

METHODS: Six-week-old male F344 rats were injected intraperitoneally with 20 mg/kg of doxorubicin or saline. Once the treatment was administered, both groups of animals were fasted with no food or water until sacrifice 24 h posttreatment.

RESULTS: Doxorubicin treatment affected neither the proteome lysine acetylation status nor the expression of sirtuin 1, sirtuin 3, SOD1, or SOD2 in soleus of fasted animals. Doxorubicin treatment also did not affect the expression or activation of procaspase-1, procaspase-8, procaspase-9, or procaspase-12.

CONCLUSION: We suggest that doxorubicin does not exert a direct effect on these catabolic parameters in skeletal muscle in vivo.

Author List

Dirks-Naylor AJ, Tran NT, Yang S, Mabolo R, Kouzi SA

Author

Sendra Yang PharmD Assistant Clinical Professor in the School of Pharmacy department at Medical College of Wisconsin



View this publication's entry at the Pubmed website PMID: 23529675
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