Immunomodulatory and antitumor activity of Piper longum Linn. and piperine. J Ethnopharmacol 2004 Feb;90(2-3):339-46
Date
03/12/2004Pubmed ID
15013199DOI
10.1016/j.jep.2003.10.016Scopus ID
2-s2.0-0842283177 (requires institutional sign-in at Scopus site) 409 CitationsAbstract
Alcoholic extract of the fruits of the plant Piper longum and its component piperine was studied for their immunomodulatory and antitumor activity. Alcoholic extract of the fruits was 100% toxic at a concentration of 500 microg/ml to Dalton's lymphoma ascites (DLA) cells and 250 microg/ml to Ehrlich ascites carcinoma (EAC) cells. Piperine was found to be cytotoxic towards DLA and EAC cells at a concentration of 250 microg/ml. Alcoholic extract and piperine was also found to produce cytotoxicity towards L929 cells in culture at a concentration of 100 and 50 microg/ml, respectively. Administration of alcoholic extract of Piper longum (10 mg/dose/animal) as well as piperine (1.14 mg/dose/animal) could inhibit the solid tumor development in mice induced with DLA cells and increase the life span of mice bearing Ehrlich ascites carcinoma tumor to 37.3 and 58.8%, respectively. Administration of Piper longum extract and piperine increased the total WBC count to 142.8 and 138.9%, respectively, in Balb/c mice. The number of plaque forming cells also enhanced significantly by the administration of the extract (100.3%) and piperine (71.4%) on 5th day after immunization. Bone marrow cellularity and alpha-esterase positive cells were also increased by the administration of Piper longum extract and piperine.
Author List
Sunila ES, Kuttan GAuthor
Sunila Pradeep PhD Associate Professor in the Obstetrics and Gynecology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adjuvants, ImmunologicAlkaloids
Animals
Antineoplastic Agents, Phytogenic
Benzodioxoles
Carcinoma, Ehrlich Tumor
Mice
Mice, Inbred BALB C
Piper
Piperidines
Plant Extracts
Polyunsaturated Alkamides
Sheep
Xenograft Model Antitumor Assays
