Faculty Collaboration Database

In vitro analysis of yeast mitochondrial protein import. Curr Protoc Cell Biol 2007 Mar;Chapter 11:Unit 11.19

Date

01/30/2008

Pubmed ID

18228496

DOI

10.1002/0471143030.cb1119s34

Scopus ID

2-s2.0-39349104703 (requires institutional sign-in at Scopus site)   11 Citations

Abstract

This unit describes methods for importing in vitro-translated or recombinant proteins into isolated yeast mitochondria and for exporting mitochondrial proteins translated in the yeast mitochondrial matrix into the inner mitochondrial membrane. The methods use mitochondria isolated from yeast cells and mitochondrial protein precursors derived from an in vitro transcription/translation reaction or purified from an E. coli recombinant protein expression system. The described translocation assays can be used to determine whether a protein is targeted to mitochondria and its location within the mitochondrion. It can also be used to study the import mechanism and to investigate mitochondrial matrix translation of proteins and their export.

Author List

Stuart RA, Koehler CM

Author

Rosemary Stuart PhD Professor in the Biology department at Marquette University

MESH terms used to index this publication - Major topics in bold

Electrophoresis, Polyacrylamide Gel
Escherichia coli
Genes, Mitochondrial
Indicators and Reagents
Mitochondria
Mitochondrial Membrane Transport Proteins
Mitochondrial Membranes
Mitochondrial Proteins
Protein Biosynthesis
Protein Sorting Signals
Protein Transport
Recombinant Fusion Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription, Genetic