Faculty Collaboration Database

Explant culture of rat esophagus in a chemically defined medium. In Vitro 1981 Aug;17(8):681-8

Date

08/01/1981

Pubmed ID

7327597

DOI

10.1007/BF02628403

Scopus ID

2-s2.0-0019781063 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Esophagus from adult male CDF rats was cultured for a period of 28 d in CMRL-1066 medium supplemented with pyruvic acid, HEPES buffer, beta-retinyl acetate, and antibiotics. Morphological, radioautographic, and biochemical studies indicated that the survival of the tissue in serum-free medium was equivalent to that in medium containing 5% heat-inactivated fetal bovine serum. There was a relatively constant uptake of [3H]thymidine into DNA and [3H]leucine into protein of the esophageal explants during the incubation. Only the basal cells of the epithelium incorporated [3H]thymidine into their nuclei. The normal morphology of the tissue was preserved when the explants were maintained at both 37 and 30 degrees C, and in either 50 or 20 % 02. Ninety-five percent O2 was highly toxic to the cells of the explants. This culture system should be suitable for a variety of investigations in esophageal cell differentiation and carcinogenesis.

Author List

Stoner GD, Pettis W, Haugen A, Jackson F, Harris CC

MESH terms used to index this publication - Major topics in bold

Animals
Cell Survival
Culture Media
Culture Techniques
DNA
Esophagus
Male
Microscopy, Electron
Protein Biosynthesis
Rats