Cutting Edge: Check Your Mice-A Point Mutation in the Ncr1 Locus Identified in CD45.1 Congenic Mice with Consequences in Mouse Susceptibility to Infection. J Immunol 2018 Mar 15;200(6):1982-1987
Date
02/15/2018Pubmed ID
29440507Pubmed Central ID
PMC5840015DOI
10.4049/jimmunol.1701676Scopus ID
2-s2.0-85044713946 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
B6.SJL-Ptprca Pepcb /Boy (CD45.1) mice have been used in hundreds of congenic competitive transplants, with the presumption that they differ from C57BL/6 mice only at the CD45 locus. In this study, we describe a point mutation in the natural cytotoxicity receptor 1 (Ncr1) locus fortuitously identified in the CD45.1 strain. This point mutation was mapped at the 40th nucleotide of the Ncr1 locus causing a single amino acid mutation from cysteine to arginine at position 14 from the start codon, resulting in loss of NCR1 expression. We found that these mice were more resistant to CMV due to a hyper innate IFN-γ response in the absence of NCR1. In contrast, loss of NCR1 increased susceptibility to influenza virus, a result that is consistent with the role of NCR1 in the recognition of influenza Ag, hemagglutinin. This work sheds light on potential confounding experimental interpretation when this congenic strain is used as a tool for tracking lymphocyte development.
Author List
Jang Y, Gerbec ZJ, Won T, Choi B, Podsiad A, B Moore B, Malarkannan S, Laouar YAuthor
Subramaniam Malarkannan PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Ly
Hemagglutinins
Immunity, Innate
Interferon-gamma
Leukocyte Common Antigens
Mice
Mice, Congenic
Mice, Inbred C57BL
Natural Cytotoxicity Triggering Receptor 1
Orthomyxoviridae
Orthomyxoviridae Infections
Point Mutation