Elimination of channel-forming activity by insertional inactivation of the p66 gene in Borrelia burgdorferi. FEMS Microbiol Lett 2007 Jan;266(2):241-9
Date
01/20/2007Pubmed ID
17233736DOI
10.1111/j.1574-6968.2006.00529.xScopus ID
2-s2.0-33845650737 (requires institutional sign-in at Scopus site) 33 CitationsAbstract
P66 is a chromosomally encoded 66-kDa integral outer membrane protein of the Lyme disease agent Borrelia burgdorferi exhibiting channel-forming activity. Herein, we inactivated and subsequently complemented the p66 gene in the B31-A (WT) strain. The P66 protein was also inactivated in two other channel-forming protein mutant strains, P13-18 (Deltap13) and Deltabba01, and then compared with the channel-forming activities of wild-type and various p66 mutant strains. We further investigated the ion-selectivity of native, purified P66. In conclusion, we show that the porin activity of P66 is eliminated by insertional inactivation and that this activity can be rescued by gene complementation.
Author List
Pinne M, Thein M, Denker K, Benz R, Coburn J, Bergström SAuthor
Jenifer Coburn PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Bacterial ProteinsBorrelia burgdorferi
Genetic Complementation Test
Immunoblotting
Ion Channels
Membrane Proteins
Mutagenesis, Insertional
Mutation
Porins