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Formation of smooth muscle alpha actin filaments in CD34+ bone marrow cells on arterial elastic laminae: potential role of SH2 domain-containing protein tyrosine phosphatase-1. Matrix Biol 2008 May;27(4):282-94 PMID: 18258420

Pubmed ID

18258420

DOI

10.1016/j.matbio.2008.01.001

Abstract

Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs. Here, we investigated the role of elastic laminae in regulating the formation of SM alpha actin filaments in mouse CD34+ bone marrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process. Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM alpha actin filaments. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 in mediating the formation of SM alpha actin filaments. In cell culture tests, elastic laminae, but not collagen matrix, stimulated the formation of SM alpha actin filaments in CD34+ cells. The phosphatase SHP-1 mediated the stimulatory effect of elastic laminae. The interaction of CD34+ cells with elastic laminae, but not with collagen matrix, induced activation of SHP-1. The suppression of SHP-1 by transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown significantly reduced the formation of SM alpha actin filaments in CD34+ cells cultured on elastic laminae. The in vitro observations were confirmed by using an in vivo model of implantation of elastic lamina and collagen matrix scaffolds into the aorta. These observations suggest that elastic laminae stimulate the formation of SM alpha actin filaments in CD34+ bone marrow cells and SHP-1 mediates the stimulatory effect of elastic laminae.

Author List

Liu SQ, Tefft BJ, Zhang A, Zhang LQ, Wu YH

Author

Brandon J. Tefft PhD Assistant Professor in the Biomedical Engineering department at Medical College of Wisconsin




Scopus

2-s2.0-43049095134   5 Citations

MESH terms used to index this publication - Major topics in bold

Actin Cytoskeleton
Actins
Animals
Antigens, CD34
Arteries
Biomarkers
Bone Marrow Cells
Cells, Cultured
Collagen
Elasticity
Extracellular Matrix
Male
Mice
Muscle, Smooth
Phosphorylation
Protein Tyrosine Phosphatase, Non-Receptor Type 6
RNA, Small Interfering
jenkins-FCD Prod-332 f92a19b0ec5e8e1eff783fac390ec127e367c2b5