Use Of Ankle Immobilization In Evaluating Treatments To Promote Longitudinal Muscle Growth In Mice. Muscle Nerve 2018 Nov;58(5):718-725
Date
07/08/2018Pubmed ID
29981243Pubmed Central ID
PMC6246800DOI
10.1002/mus.26296Scopus ID
2-s2.0-85054337476 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
INTRODUCTION: Difficulty in modeling congenital contractures (deformities of muscle-tendon unit development that include shortened muscles and lengthened tendons) has limited research of new treatments.
METHODS: Early immobilization of the ankle in prepuberal mice was used to produce deformities similar to congenital contractures. Stretch treatment, electrostimulation, and local intramuscular injection of a follistatin analog (FST-288) were assessed as therapeutic interventions for these deformities.
RESULTS: Ankle immobilization at full plantarflexion and 90 ° created tendon lengthening and muscle shortening in the tibialis anterior and soleus. Stretch treatment produced minimal evidence for longitudinal muscle growth and electrostimulation provided no additional benefit. Stretch treatment with FST-288 produced greater longitudinal muscle growth and less tendon lengthening, constituting the best treatment response.
DISCUSSION: Ankle immobilization recapitulates key morphologic features of congenital contracture, and these features can be mitigated by a combination of stretch and pharmacological approaches that may be useful in patients. Muscle Nerve 58: 718-725, 2018.
Author List
Tinklenberg J, Beatka M, Bain JLW, Siebers EM, Meng H, Pearsall RS, Lawlor MW, Riley DAAuthor
Michael W. Lawlor MD, PhD Adjunct Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnkle Injuries
Biomechanical Phenomena
Disease Models, Animal
Electric Stimulation Therapy
Female
Follistatin
Immobilization
Male
Mice
Muscle Contraction
Muscle, Skeletal
Sarcomeres
Splints
Statistics, Nonparametric
Tendons
Time Factors
