Faculty Collaboration Database

The contribution of endogenous glutamatergic input in the ventral respiratory column to respiratory rhythm. Respir Physiol Neurobiol 2019 Feb;260:37-52

Date

12/07/2018

Pubmed ID

30502519

Pubmed Central ID

PMC6397772

DOI

10.1016/j.resp.2018.11.011

Scopus ID

2-s2.0-85058568248 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

Glutamate is the predominant excitatory neurotransmitter in the ventral respiratory column; however, the contribution of glutamatergic excitation in the individual subregions to respiratory rhythm generation has not been fully delineated. In an adult, in vivo, decerebrate rabbit model during conditions of mild hyperoxic hypercapnia we blocked glutamatergic excitation using the receptor antagonists 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) and d(-)-2-amino-5-phosphonopentanoic acid (AP5). Disfacilitation of the preBötzinger Complex caused a decrease in inspiratory and expiratory duration as well as peak phrenic amplitude and ultimately apnea. Disfacilitation of the Bötzinger Complex caused a decrease in inspiratory and expiratory duration; subsequent disfacilitation of the preBötzinger Complex resulted in complete loss of the respiratory pattern but maintained tonic inspiratory activity. We conclude that glutamatergic drive to the preBötzinger Complex is essential for respiratory rhythm generation. Glutamatergic drive to the Bötzinger Complex significantly affects inspiratory and expiratory phase duration. Bötzinger Complex neurons are responsible for maintaining the silent expiratory phase of the phrenic neurogram.

Author List

Cook-Snyder DR, Miller JR, Navarrete-Opazo AA, Callison JJ, Peterson RC, Hopp FA, Stuth EAE, Zuperku EJ, Stucke AG

Authors

Denise R. Cook-Snyder PhD Associate Professor in the Physiology department at Medical College of Wisconsin
Astrid G. Stucke MD Professor in the Anesthesiology department at Medical College of Wisconsin
Eckehard A. Stuth MD Professor in the Anesthesiology department at Medical College of Wisconsin
Edward J. Zuperku PhD Professor in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Analysis of Variance
Animals
Brain Mapping
Excitatory Amino Acid Agonists
Excitatory Amino Acid Antagonists
Female
Glutamic Acid
Male
Microinjections
Neurons
Periodicity
Phrenic Nerve
Rabbits
Respiration
Respiratory Center
Respiratory Mechanics