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Persistence of DNA in a Kidney-Liver Transplant Recipient. Am J Trop Med Hyg 2019 Mar;100(3):584-587

Date

01/11/2019

Pubmed ID

30628570

Pubmed Central ID

PMC6402933

DOI

10.4269/ajtmh.18-0752

Scopus ID

2-s2.0-85062624381   1 Citation

Abstract

Mitochondrial genome analysis of suggests that diversity of intermediate host snails drove intra-species divergence during its expansion in Asia. We applied the knowledge of this genomic variation to study an unusual patient we recently diagnosed with schistosomiasis. The patient had not visited any schistosomiasis-endemic countries for more than 35 years and had no idea where she became infected. Unusual clinical features of this patient included the absence of egg granulomas in tissue and persistent noncalcified eggs despite multiple praziquantel (PZQ) treatments over 7 years. A digital droplet polymerase chair reaction (PCR) assay that specifically targets the schistosome 1,4 dihydronicotinamide adenine dinucleotide-1 (NADH1) dehydrogenase-1 mitochondrial gene successfully amplified parasite DNA extracted from colon biopsies. DNA sequence analysis of parasite DNA revealed that it was a Philippine strain of . Future molecular studies using stored DNA from patients such as this may provide new insight into why some persons do not respond well to PZQ treatment.

Author List

Kron M, Gordon C, Bauers T, Lu Z, Mahatme S, Shah J, Saeian K, McManus DP

Authors

Michael Kron MD Director, Professor in the Medicine department at Medical College of Wisconsin
Sheran Mahatme DO Assistant Professor in the Medicine department at Medical College of Wisconsin
Kia Saeian MD Professor in the Medicine department at Medical College of Wisconsin




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