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Multicolour spectral karyotyping of mouse chromosomes. Nat Genet 1996 Nov;14(3):312-5

Date

11/01/1996

Pubmed ID

8896561

DOI

10.1038/ng1196-312

Abstract

Murine models of human carcinogenesis are exceedingly valuable tools to understand genetic mechanisms of neoplastic growth. The identification of recurrent chromosomal rearrangements by cytogenetic techniques serves as an initial screening test for tumour specific aberrations. In murine models of human carcinogenesis, however, karyotype analysis is technically demanding because mouse chromosomes are acrocentric and of similar size. Fluorescence in situ hybridization (FISH) with mouse chromosome specific painting probes can complement conventional banding analysis. Although sensitive and specific, FISH analyses are restricted to the visualization of only a few mouse chromosomes at a time. Here we apply a novel imaging technique that we developed recently for the visualization of human chromosomes to the simultaneous discernment of all mouse chromosomes. The approach is based on spectral imaging to measure chromosome-specific spectra after FISH with differentially labelled mouse chromosome painting probes. Utilizing a combination of Fourier spectroscopy, CCD-imaging and conventional optical microscopy, spectral imaging allows simultaneous measurement of the fluorescence emission spectrum at all sample points. A spectrum-based classification algorithm has been adapted to karyotype mouse chromosomes. We have applied spectral karyotyping (SKY) to chemically induced plasmocytomas, mammary gland tumours from transgenic mice overexpressing the c-myc oncogene and thymomas from mice deficient for the ataxia telangiectasia (Atm) gene. Results from these analyses demonstrate the potential of SKY to identify complex chromosomal aberrations in mouse models of human carcinogenesis.

Author List

Liyanage M, Coleman A, du Manoir S, Veldman T, McCormack S, Dickson RB, Barlow C, Wynshaw-Boris A, Janz S, Wienberg J, Ferguson-Smith MA, Schröck E, Ried T

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Ataxia Telangiectasia Mutated Proteins
Cell Cycle Proteins
Chromosome Aberrations
Chromosomes
DNA-Binding Proteins
Disease Models, Animal
Genes, myc
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Mice, Transgenic
Neoplasms
Plasmacytoma
Protein-Serine-Threonine Kinases
Proteins
Tumor Suppressor Proteins
jenkins-FCD Prod-398 336d56a365602aa89dcc112f077233607d6a5abc