Faculty Collaboration Database

The uptake of pristane (2,6,10,14-tetramethylpentadecane) into phospholipid bilayers as assessed by NMR, DSC, and tritium labeling methods. Biochim Biophys Acta 1991 Dec 09;1070(2):409-18

Date

12/09/1991

Pubmed ID

1764453

DOI

10.1016/0005-2736(91)90081-i

Scopus ID

2-s2.0-0026038368 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Unilamellar dioleoylphosphatidylcholine (DOPC) liposomes (250 microM) incorporated 2 mol% of [3H]pristane at 37 degrees C after addition of 50 microM pristane solubilized with beta-cyclodextrin. Conventional solubilization in dimethyl sulphoxide resulted in much lower uptake. Premixing of perdeuterated pristane with DOPC and dipalmitoylphosphatidylcholine (DPPC) prior to the formation of multilamellar liposomes resulted in homogeneous incorporation of up to 5 mol% pristane at 22 degrees C and 50 degrees C, respectively, as observed by 2H-NMR. Lipid order parameters measured by 31P and 2H-NMR remained unchanged after pristane uptake. Pristane induced the transformation of part of the dioleoylphosphatidylethanolamine (DOPE)/DOPC (3:1, mol/mol) liquid crystalline lamellar phase into an inverse hexagonal phase. 5 mol% pristane in DPPC bilayers decreased the midpoint of the main phase transition temperature of DPPC from 41.5 degrees C to 40.9 degrees C. Upon cooling in the temperature range from 41 degrees C to 36 degrees C, pristane was either displaced from the DPPC bilayer or the mode of incorporation changed. These results may aid in defining the mechanisms whereby pristane, an isoprenoid C19-isoalkane, induces plasmacytomagenesis in mice.

Author List

Gawrisch K, Janz S

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

1,2-Dipalmitoylphosphatidylcholine
Calorimetry, Differential Scanning
Carcinogens
Lipid Bilayers
Magnetic Resonance Spectroscopy
Models, Biological
Phosphatidylcholines
Phosphatidylethanolamines
Phospholipids
Radioisotope Dilution Technique
Terpenes
Tritium