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A new method for delivering alkanes to mammalian cells: preparation and preliminary characterization of an inclusion complex between beta-cyclodextrin and pristane (2,6,10,14-tetramethylpentadecane). Toxicology 1991;69(3):301-15

Date

01/01/1991

Pubmed ID

1949053

DOI

10.1016/0300-483x(91)90189-8

Abstract

Pristane (2,6,10,14-tetramethylpentadecane) is an isoalkane which induces plasma cell tumorigenesis in genetically susceptible strains of mice. Attempts to study the biological activity of pristane on cells in vitro have been hindered by the extreme hydrophobicity and hence complete immiscibility of the compound in aqueous cell culture media. In comparing different solubilization protocols such as using organic solvents, liposomes, and molecular encapsulation into beta-cyclodextrin (beta-CyD), it was found that beta-CyD/pristane inclusion complexes were optimal for delivery of the hydrocarbon to cells. After solubilization in beta-CyD, pristane was cytotoxic (51Cr release assay) to murine B lymphocyte lines in culture (P388, NSF-1, and SJL-4) and inhibited the lipopolysaccharide-induced stimulation of splenic B lymphocyte proliferation and blast formation (Coulter counter analysis) when added in the micromolar concentration range. Moreover, sub-toxic concentrations of beta-CyD/pristane inclusion complexes were found to have a small but reproducible mitogenic effect [( 3H]thymidine incorporation) on SJL-4 and 308 (murine initiated keratinocyte) cells in culture but not on P388 cells. The results indicate that molecular encapsulation of pristane into beta-CyD provides a new and effective method for delivering low concentrations of alkanes to mammalian cells in vitro.

Author List

Janz S, Shacter E

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Alkanes
Animals
B-Lymphocytes
Cell Death
Cell Division
Cell Line
Chemical Phenomena
Chemistry, Physical
Chromium Radioisotopes
Cyclodextrins
Liposomes
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Solubility
Terpenes
Thymidine
Tritium
Tumor Cells, Cultured
beta-Cyclodextrins
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