Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes. Am J Physiol Heart Circ Physiol 2008 Nov;295(5):H2178-87
Date
10/07/2008Pubmed ID
18835915Pubmed Central ID
PMC2614582DOI
10.1152/ajpheart.00748.2008Scopus ID
2-s2.0-57049176060 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
Adult rat cardiac myocytes typically display a phenotypic response to cytokines manifested by low or no increases in nitric oxide (NO) production via inducible NO synthase (iNOS) that distinguishes them from other cell types. To better characterize this response, we examined the expression of tetrahydrobiopterin (BH4)-synthesizing and arginine-utilizing genes in cytokine-stimulated adult cardiac myocytes. Intracellular BH4 and 7,8-dihydrobiopterin (BH2) and NO production were quantified. Cytokines induced GTP cyclohydrolase and its feedback regulatory protein but with deficient levels of BH4 synthesis. Despite the induction of iNOS protein, cytokine-stimulated adult cardiac myocytes produced little or no increase in NO versus unstimulated cells. Western blot analysis under nonreducing conditions revealed the presence of iNOS monomers. Supplementation with sepiapterin (a precursor of BH4) increased BH4 as well as BH2, but this did not enhance NO levels or eliminate iNOS monomers. Similar findings were confirmed in vivo after treatment of rat cardiac allograft recipients with sepiapterin. It was found that expression of dihydrofolate reductase, required for full activity of the salvage pathway, was not detected in adult cardiac myocytes. Thus, adult cardiac myocytes have a limited capacity to synthesize BH4 after cytokine stimulation. The mechanisms involve posttranslational factors impairing de novo and salvage pathways. These conditions are unable to support active iNOS protein dimers necessary for NO production. These findings raise significant new questions about the prevailing understanding of how cytokines, via iNOS, cause cardiac dysfunction and injury in vivo during cardiac inflammatory disease states since cardiac myocytes are not a major source of high NO production.
Author List
Ionova IA, Vásquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GMAuthor
Jeannette M. Vasquez-Vivar PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Alcohol OxidoreductasesAnimals
Arginase
Cells, Cultured
Cytokines
GTP Cyclohydrolase
Heart Transplantation
Intracellular Signaling Peptides and Proteins
Male
Myocytes, Cardiac
Nitric Oxide
Nitric Oxide Synthase Type II
Phosphorus-Oxygen Lyases
Proteins
Pterins
RNA, Messenger
Rats
Rats, Inbred Lew
Rats, Inbred WF
Rats, Sprague-Dawley
Tetrahydrofolate Dehydrogenase
Time Factors