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Mesh term Fluorescence Recovery After Photobleaching

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Fluorometry

Description

A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).



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jenkins-FCD Prod-310 bff9d975ec7f2d302586822146c2801dd4449aad