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Production of volatile nitrogenous compounds from the degradation of streptomycin by Pseudomonas maltophilia. J Bacteriol 1973 Dec;116(3):1267-72

Date

12/01/1973

Pubmed ID

4752939

Pubmed Central ID

PMC246483

DOI

10.1128/jb.116.3.1267-1272.1973

Scopus ID

2-s2.0-0015759497   7 Citations

Abstract

Ammonia, methylamine, and pyridine were detected in broth filtrates of a streptomycin-degrading strain of Pseudomonas maltophilia during growth on streptomycin as a sole carbon and nitrogen source. Ammonia and methylamine, quantitatively measured by conversion to chromophores with picryl sulfonic acid, were found to accumulate in broth, whereas pyridine concentration increased in the early stages of streptomycin degradation and then decreased as the degradation of the antibiotic neared completion. Exogenous pyridine was metabolized by washed-cell suspensions. Use of N-streptomycin-methyl-(14)C showed that the methylamine arose from the N-l-glucosamine-methyl moiety of streptomycin. Methylamine was an end product and was not further metabolized by cells.

Author List

Fenton JJ, Harsch HH, Klein D

Author

Harold H. Harsch MD Professor in the Psychiatry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Ammonia
Autoanalysis
Carbon Radioisotopes
Chromatography, Gas
Chromatography, Paper
Chromatography, Thin Layer
Culture Media
Methionine
Methylamines
Pseudomonas
Pyridines
Spectrophotometry, Ultraviolet
Streptomycin