Production of volatile nitrogenous compounds from the degradation of streptomycin by Pseudomonas maltophilia. J Bacteriol 1973 Dec;116(3):1267-72
Date
12/01/1973Pubmed ID
4752939Pubmed Central ID
PMC246483DOI
10.1128/jb.116.3.1267-1272.1973Scopus ID
2-s2.0-0015759497 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Ammonia, methylamine, and pyridine were detected in broth filtrates of a streptomycin-degrading strain of Pseudomonas maltophilia during growth on streptomycin as a sole carbon and nitrogen source. Ammonia and methylamine, quantitatively measured by conversion to chromophores with picryl sulfonic acid, were found to accumulate in broth, whereas pyridine concentration increased in the early stages of streptomycin degradation and then decreased as the degradation of the antibiotic neared completion. Exogenous pyridine was metabolized by washed-cell suspensions. Use of N-streptomycin-methyl-(14)C showed that the methylamine arose from the N-l-glucosamine-methyl moiety of streptomycin. Methylamine was an end product and was not further metabolized by cells.
Author List
Fenton JJ, Harsch HH, Klein DAuthor
Harold H. Harsch MD Professor in the Psychiatry and Behavioral Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AmmoniaAutoanalysis
Carbon Radioisotopes
Chromatography, Gas
Chromatography, Paper
Chromatography, Thin Layer
Culture Media
Methionine
Methylamines
Pseudomonas
Pyridines
Spectrophotometry, Ultraviolet
Streptomycin