Faculty Collaboration Database

Histological evaluation of the effects of angiotensin peptides on wound repair in diabetic mice. Exp Dermatol 2003 Dec;12(6):784-90

Date

01/13/2004

Pubmed ID

14714558

DOI

10.1111/j.0906-6705.2003.00087.x

Scopus ID

2-s2.0-0347062408 (requires institutional sign-in at Scopus site)   44 Citations

Abstract

Recent studies have shown that angiotensin peptides accelerate dermal repair. Histological observation of samples taken at the termination of studies showed that the wounds treated with peptides were mature and organized by day 25 after full thickness excision in diabetic mice. However, the mechanisms by which this acceleration occurs has not been determined. In the experiments described here, the effect of angiotensin peptides (AII, A(1-7) and NorLeu (3)-A(1-7) on the quality of the healing wound was evaluated histologically. Administration of the peptides accelerated collagen deposition, re-epithelialization and new blood vessels formation. By day 4, the percentage of the wound with collagen increased two- to six-fold depending upon the peptide. The increase by angiotensin peptides continued throughout the experimental period. On days 4 and 7 9 (only) after injury, exposure to angiotensin peptides increased the number of blood vessels at wound site two-to three-fold. Finally, the percentage of the wound site covered with new epithelium increased after administration of angiotensin peptides. Re-epithelialization was observed as early as day 4 in wounds treated ith angiotensin peptides. The increase was greater at later time points (up to 8-fold ar day 14 with NorLeu(3)-A(1-7) had an increase in neutrophils and macrophages on day 4 after wounding. Overall, administration of these peptides resulted in a healing site that was more mature, including reorganization of the collagen into a basket-weave appearance. Further, these studies confirm the superiority of NorLeu(3)-A(1-7) to AII and A(1-7) in wound healing evaluated at a microscopic level.

Author List

Rodgers KE, Roda N, Felix JE, Espinoza T, Maldonado S, diZerega G

Author

Juan Felix MD Vice Chair, Director, Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Angiotensins
Animals
Cell Division
Collagen
Diabetes Mellitus, Experimental
Fibroblasts
Leukocytes
Mice
Mice, Inbred C57BL
Peptides
Placebos
Time Factors
Wound Healing