Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves. Tissue Eng Part C Methods 2015 Sep;21(9):987-94
Date
04/29/2015Pubmed ID
25915105Pubmed Central ID
PMC4553370DOI
10.1089/ten.TEC.2015.0011Scopus ID
2-s2.0-84940101539 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.
Author List
James IA, Yi T, Tara S, Best CA, Stuber AJ, Shah KV, Austin BF, Sugiura T, Lee YU, Lincoln J, Trask AJ, Shinoka T, Breuer CKAuthor
Joy Lincoln PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsHeart Transplantation
Heart Valve Prosthesis
Heart Valves
Heart Ventricles
Hemodynamics
Mice, Inbred C57BL
Models, Animal
Pressure
Pulmonary Valve
Tissue Engineering
Ultrasonography