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The role of neural precursor cells and self assembling peptides in nerve regeneration. J Otolaryngol Head Neck Surg 2013 Dec 19;42(1):60

Date

12/20/2013

Pubmed ID

24351041

Pubmed Central ID

PMC3878249

DOI

10.1186/1916-0216-42-60

Scopus ID

2-s2.0-84896987191   6 Citations

Abstract

OBJECTIVE: Cranial nerve injury involves loss of central neural cells in the brain stem and surrounding support matrix, leading to severe functional impairment. Therapeutically targeting cellular replacement and enhancing structural support may promote neural regeneration. We examined the combinatorial effect of neural precursor cells (NPC) and self assembling peptide (SAP) administration on nerve regeneration.

METHODS: Nerve injury was induced by clip compression of the rodent spinal cord. SAPs were injected immediately into the injured cord and NPCs at 2 weeks post-injury. Behavioral analysis was done weekly and rats were sacrificed at 11 weeks post injury. LFB-H&E staining was done on cord tissue to assess cavitation volume. Motor evoked potentials (MEP) were measured at week 11 to assess nerve conduction and Kaplan Meier curves were created to compare survival estimates.

RESULTS: NPCs and SAPs were distributed both caudal and rostral to the injury site. Behavioral analysis showed that SAP + NPC transplantation significantly improved locomotor score p <0.03) and enhanced survival (log rank test, p = 0.008) compared to control. SAP + NPC treatment also improved nerve conduction velocity (p = 0.008) but did not affect cavitation volume (p = 0.73).

CONCLUSION: Combinatorial NPC and SAP injection into injured nerve tissue may enhance neural repair and regeneration.

Author List

Zhao X, Yao GS, Liu Y, Wang J, Satkunendrarajah K, Fehlings M

Author

Kajana Satkunendrarajah PhD Assistant Professor in the Neurosurgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cell Transplantation
Evoked Potentials, Motor
Female
Kaplan-Meier Estimate
Nerve Regeneration
Neural Stem Cells
Neurons
Peptides
Pilot Projects
Rats
Rats, Wistar
Spinal Cord Injuries
Tissue Scaffolds