Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Immunoglobulin G (IgG) attenuates neuroinflammation and improves neurobehavioral recovery after cervical spinal cord injury. J Neuroinflammation 2012 Sep 21;9:224



Pubmed ID


Pubmed Central ID




Scopus ID

2-s2.0-84866478622   53 Citations


BACKGROUND: Evidence suggests that the inflammatory events in the acute phase of spinal cord injury (SCI) exacerbate the initial trauma to the cord leading to poor functional recovery. As a result, minimizing the detrimental aspects of the inflammatory response after SCI is a promising treatment strategy. In this regard, immunoglobulin G (IgG) from pooled human serum is a promising treatment candidate. Due to its putative, though poorly characterized immuno-modulatory effects, IgG has been used clinically to treat neuroinflammatory disorders such as Guillain-Barré syndrome, but its effects in neurotrauma remain largely unexplored.

METHODS: This study examines the potential neuroprotective effects of IgG in a well-characterized cervical model of SCI. Female Wistar rats were subject to moderate-severe clip compression injury at the C7-T1 level. IgG (0.4 g/kg) or saline was injected intravenously to randomly selected animals at 15 min post SCI. At several time points post SCI, biochemical assays, histology and immunohistochemistry analyses, and neurobehavioral assessments were used to examine the neuroprotective effects of IgG at the molecular, cellular, and neurobehavioral levels.

RESULTS: We found that intravenous treatment of IgG following acute clip-compression SCI at C7-T1 significantly reduced two important inflammatory cytokines: interleukin (IL)-1β and IL-6. This early reduction in pro-inflammatory signaling was associated with significant reductions in neutrophils in the spinal cord and reductions in the expression of myeloperoxidase and matrix metalloproteinase-9 in the injured spinal cord at 24 h after SCI. These beneficial effects of IgG were associated with enhanced tissue preservation, improved neurobehavioral recovery as measured by the BBB and inclined plane tests, and enhanced electrophysiological evidence of central axonal conduction as determined by motor-evoked potentials.

CONCLUSION: The findings from this study indicate that IgG is a novel immuno-modulatory therapy which shows promise as a potential treatment for SCI.

Author List

Nguyen DH, Cho N, Satkunendrarajah K, Austin JW, Wang J, Fehlings MG


Kajana Satkunendrarajah PhD Assistant Professor in the Neurosurgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Analysis of Variance
Behavior, Animal
Calcium-Binding Proteins
Disease Models, Animal
Drug Administration Routes
Enzyme-Linked Immunosorbent Assay
Evoked Potentials, Motor
Gene Expression Regulation
Glial Fibrillary Acidic Protein
Immunoglobulin G
Matrix Metalloproteinase 9
Microfilament Proteins
Neuroprotective Agents
Neutrophil Infiltration
Psychomotor Performance
Rats, Wistar
Recovery of Function
Spinal Cord Injuries
Tissue Distribution