Faculty Collaboration Database

UV-induced changes in cell cycle and gene expression within rabbit lens epithelial cells. Photochem Photobiol 1996 Jan;63(1):79-85

Date

01/01/1996

Pubmed ID

8577869

DOI

10.1111/j.1751-1097.1996.tb02995.x

Scopus ID

2-s2.0-0030041844 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Damage to lens epithelial cells is a probable initiation process in cataract formation mediated by UV radiation. In these experiments, we investigated the effects of exposure to 254 nm radiation on cell cycle progression and gene expression in the rabbit lens epithelial cell line N/N1003A. The RNA was harvested at various times following exposure to UV (254 nm) radiation and analyzed by dot-blot and northern blot hybridizations. These results revealed that during the first 6 h following exposure of the cells to UV, there was, associated with decreasing dose, a decrease in accumulation of transcripts specific for histones H3 and H4 and an increase in the mRNA encoding protein kinase C and beta- and gamma-actin. Using flow cytometry, we detected an accumulation of cells in G1/S phase of the cell cycle 1 h following exposure to 254 nm radiation. The observed changes in gene expression, especially the decreased accumulation of histone transcripts reported here, may play a role in UV-induced inhibition of cell cycle progression.

Author List

Sidjanin D, Grdina D, Woloschak GE

Author

Danielle Sidjanin Maier PhD, MSN APP Supervisor Inpatient 2 in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Actins
Animals
Cell Cycle
Cell Line
Cell Survival
Epithelial Cells
Epithelium
Gene Expression Regulation
Histones
Lens, Crystalline
Protein Kinase C
Rabbits
Ultraviolet Rays