Investigating the Potential of Amnion-Based Scaffolds as a Barrier Membrane for Guided Bone Regeneration. Langmuir 2015 Aug 11;31(31):8642-53
Date
07/15/2015Pubmed ID
26158559DOI
10.1021/acs.langmuir.5b02362Scopus ID
2-s2.0-84938703785 (requires institutional sign-in at Scopus site) 45 CitationsAbstract
Guided bone regeneration is a new concept of large bone defect therapy, which employs a barrier membrane to afford a protected room for osteogenesis and prevent the invasion of fibroblasts. In this study, we developed a novel barrier membrane made from lyophilized multilayered acellular human amnion membranes (AHAM). After decellularization, the AHAM preserved the structural and biomechanical integrity of the amnion extracellular matrix (ECM). The AHAM also showed minimal toxic effects when cocultured with mesenchymal stem cells (MSCs), as evidenced by high cell density, good cell viability, and efficient osteogenic differentiation after 21-day culturing. The effectiveness of the multilayered AHAM in guiding bone regeneration was evaluated using an in vivo rat tibia defect model. After 6 weeks of surgery, the multilayered AHAM showed great efficiency in acting as a shield to avoid the invasion of the fibrous tissues, stabilizing the bone grafts and inducing the massive bone growth. We hence concluded that the advantages of the lyophilized multilayered AHAM barrier membrane are as follows: preservation of the structural and mechanical properties of the amnion ECM, easiness for preparation and handling, flexibility in adjusting the thickness and mechanical properties to suit the application, and efficiency in inducing bone growth and avoiding fibrous tissues invasion.
Author List
Li W, Ma G, Brazile B, Li N, Dai W, Butler JR, Claude AA, Wertheim JA, Liao J, Wang BAuthor
Bo Wang PhD Assistant Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AmnionAnimals
Bone Regeneration
Cell Survival
Cells, Cultured
Extracellular Matrix
Guided Tissue Regeneration
Humans
Rats
Rats, Sprague-Dawley
Tissue Engineering
Tissue Scaffolds