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The aqueous humor outflow pathway of zebrafish. Invest Ophthalmol Vis Sci 2009 Apr;50(4):1515-21



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Scopus ID

2-s2.0-64049089838   22 Citations


PURPOSE: The structures of the ocular anterior segment responsible for aqueous humor secretion and absorption have been well characterized in mammals. However, the underlying molecular and cellular mechanisms that regulate aqueous humor flow have remained elusive. Experimental analysis in Danio rerio, the zebrafish, is providing mechanistic insights into many cellular processes relevant to normal human physiology and disease. To facilitate studies on the molecular and cellular mechanisms of aqueous humor dynamics using this species, the authors have characterized the anatomy of aqueous secretion and outflow in adult zebrafish eyes.

METHODS: Analyses by light and transmission electron microscopy, coupled with molecular tracers of fluid flow, were used to identify and study the sites of aqueous humor secretion and absorption in adult zebrafish eyes.

RESULTS: Zebrafish eyes show aqueous humor secretion primarily from the dorsal ciliary region and outflow through a ventral canalicular network that connects with an aqueous plexus and veins of the choroidal rete.

CONCLUSIONS: Vectorial flow of zebrafish aqueous humor is in contrast to that in mammals in which secretion and absorption of aqueous humor are circumferential around and through the iridocorneal angle. However, local anatomy and ultrastructure of the tissues and cells specialized for aqueous humor dynamics in zebrafish show conservation with that of mammals. These observations suggest that zebrafish can serve as a useful genetic model to help understand the regulation and cellular basis of normal and abnormal aqueous humor dynamics in humans.

Author List

Gray MP, Smith RS, Soules KA, John SW, Link BA


Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aqueous Humor
Biological Transport, Active
Ciliary Body
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Trabecular Meshwork
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0