Sequence specificity in aflatoxin B1--DNA interactions. Proc Natl Acad Sci U S A 1983 Jan;80(1):6-10
Date
01/01/1983Pubmed ID
6218504Pubmed Central ID
PMC393298DOI
10.1073/pnas.80.1.6Scopus ID
2-s2.0-0342587337 (requires institutional sign-in at Scopus site) 119 CitationsAbstract
The activated form of aflatoxin B1 (AFB1) causes covalent modification primarily of guanine residues, leading to alkali-labile sites in DNA. A simple extension of the Maxam-Gilbert procedure for sequence analysis permits the identification of alkali-labile sites induced by AFB1 and determination of the frequency of alkali-labile AFB1 modifications at particular sites on a DNA fragment of known sequence. Using this strategy, we have investigated the influence of flanking nucleotide sequences on AFB1 modification in a number of DNA fragments of known sequence. Our results show that certain guanine residues in double-stranded DNA are preferentially attacked by AFB1 over others in a manner predictable from a knowledge of vicinal nucleotide sequences. The observed in vitro sequence specificity is independent of a number of tested parameters and is likely to occur in vivo.
Author List
Muench KF, Misra RP, Humayun MZAuthor
Ravindra P. Misra PhD, MD Associate Provost, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AflatoxinsBacteriophage phi X 174
Base Sequence
Binding Sites
DNA
Guanine
Plasmids
