Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis. J Vis Exp 2018 Mar 28(133)
Date
04/17/2018Pubmed ID
29658929Pubmed Central ID
PMC5933264DOI
10.3791/56930Scopus ID
2-s2.0-85044721273 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
In rodents, complete bile duct ligation (cBDL) of the common bile duct is an established surgical technique for studying obstructive cholestasis and bile duct proliferation. However, long-term experiments can lead to increased morbidity and mortality. In select mouse strains with underlying liver disease, meaningful comparisons can be made even with ligation of a single lobe of the liver, which can reduce animal losses and expenses. Here, we describe partial bile duct ligation (pBDL) in the mouse, in which only the left hepatic bile duct is ligated, causing biliary obstruction in the left lobe but not the remaining lobes. With careful microsurgical technique, pBDL experiments can be cost-effective, since the unligated lobe serves as an internal control to the ligated lobes, when subjected to the same conditions in the same animal. Unlike cBDL, a separate sham-operated control group is not necessary. pBDL is highly useful to directly compare localized versus systemic effects of cholestasis and other retained bile components. pBDL can also be repurposed as a novel method to investigate mechanisms related to medications and cell migration.
Author List
Yokota S, Ono Y, Nakao T, Zhang P, Michalopoulos GK, Khan ZMESH terms used to index this publication - Major topics in bold
AnimalsBile Ducts
Cholestasis
Disease Models, Animal
Ligation
Male
Mice
