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The diffusion-concentration product of oxygen in lipid bilayers using the spin-label T1 method. Biochim Biophys Acta 1981 May 06;643(2):283-91

Date

05/06/1981

Pubmed ID

6261814

Abstract

A method is described to measure the oxygen diffusion-concentration product, DO[O2], at any locus that can be probed or labeled using nitroxide radicals. The method is based on the dependence of the spin-lattice relaxation time T1 of the spin label on the bimolecular collision rate with oxygen. Strong Heisenberg exchange between spin label and oxygen contributes directly to T1 of the spin label, while dipolar interactions are negligible. Both time-domain and continuous wave saturation methods for studying T1 are considered. The method has been applied to phospholipid liposomes using fatty acid spin labels. A discontinuity in DO[O2] at the main phase transition was observed.

Author List

Subczynski WK, Hyde JS

Author

Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Diffusion
Electron Spin Resonance Spectroscopy
Lipid Bilayers
Mathematics
Oxygen
Phospholipids
Spin Labels
Temperature