Evaluation of the Coomassie Brilliant Blue G-250 method for urinary protein. Clin Chem 1983 Nov;29(11):1946-50
Date
11/01/1983Pubmed ID
6354513Scopus ID
2-s2.0-0020520301 (requires institutional sign-in at Scopus site) 105 CitationsAbstract
The Coomassie Brilliant Blue G-250 method for protein in urine has been evaluated for analytical accuracy and clinical applicability. Extremely simple to perform, the test exhibits good precision and sensitivity. The color developed per gram of protein is protein-type dependent, so no single protein standard is completely satisfactory. Color intensity is linearly related to concentration up to 1500 mg/L when used with a manual bichromatic method. Accuracy was clinically acceptable for patients with a variety of protein-losing diseases, and for patients having received renal transplants; however, the method underestimates urinary light-chain proteins. In athletes and premature neonates, we observed increased protein excretion during periods of stress. The upper reference limit for protein excretion in healthy adults is about 120 mg/24 h.
Author List
Lott JA, Stephan VA, Pritchard KA JrAuthor
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAutoanalysis
Biuret
Evaluation Studies as Topic
Female
Humans
Infant, Newborn
Infant, Premature
Kidney Transplantation
Male
Middle Aged
Multiple Myeloma
Physical Exertion
Proteins
Proteinuria
Rosaniline Dyes
Spectrophotometry
