Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish. Dev Dyn 2011 Mar;240(3):712-22
Date
02/22/2011Pubmed ID
21337469Pubmed Central ID
PMC3072245DOI
10.1002/dvdy.22567Scopus ID
2-s2.0-79951663595 (requires institutional sign-in at Scopus site) 74 CitationsAbstract
Bone morphogenic protein (BMP) signaling is fundamental to development, injury response, and homeostasis. We have developed transgenic zebrafish that report Smad-mediated BMP signaling in embryos and adults. These lines express either enhanced green fluorescent protein (eGFP), destabilized eGFP, or destabilized Kusabira Orange 2 (KO2) under the well-characterized BMP Response Element (BRE). These fluorescent proteins were found to be expressed dynamically in regions of known BMP signaling including the developing tail bud, hematopoietic lineage, dorsal eye, brain structures, heart, jaw, fins, and somites, as well as other tissues. Responsiveness to changes in BMP signaling was confirmed by observing fluorescence after activation in an hsp70:bmp2b transgenic background or by inhibition in an hsp70:nog3 background. We further demonstrated faithful reportage by the BRE transgenic lines following chemical repression of BMP signaling using an inhibitor of BMP receptor activity, dorsomorphin. Overall, these lines will serve as valuable tools to explore the mechanisms and regulation of BMP signal during embryogenesis, in tissue maintenance, and during disease.
Author List
Collery RF, Link BAAuthors
Ross F. Collery PhD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinBrian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Genetically Modified
Bone Morphogenetic Proteins
Immunohistochemistry
Microscopy, Fluorescence
Response Elements
Signal Transduction
Smad Proteins
Zebrafish
