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Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish. Dev Dyn 2011 Mar;240(3):712-22

Date

02/22/2011

Pubmed ID

21337469

Pubmed Central ID

PMC3072245

DOI

10.1002/dvdy.22567

Scopus ID

2-s2.0-79951663595   49 Citations

Abstract

Bone morphogenic protein (BMP) signaling is fundamental to development, injury response, and homeostasis. We have developed transgenic zebrafish that report Smad-mediated BMP signaling in embryos and adults. These lines express either enhanced green fluorescent protein (eGFP), destabilized eGFP, or destabilized Kusabira Orange 2 (KO2) under the well-characterized BMP Response Element (BRE). These fluorescent proteins were found to be expressed dynamically in regions of known BMP signaling including the developing tail bud, hematopoietic lineage, dorsal eye, brain structures, heart, jaw, fins, and somites, as well as other tissues. Responsiveness to changes in BMP signaling was confirmed by observing fluorescence after activation in an hsp70:bmp2b transgenic background or by inhibition in an hsp70:nog3 background. We further demonstrated faithful reportage by the BRE transgenic lines following chemical repression of BMP signaling using an inhibitor of BMP receptor activity, dorsomorphin. Overall, these lines will serve as valuable tools to explore the mechanisms and regulation of BMP signal during embryogenesis, in tissue maintenance, and during disease.

Author List

Collery RF, Link BA

Authors

Ross F. Collery PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin
Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Genetically Modified
Bone Morphogenetic Proteins
Immunohistochemistry
Microscopy, Fluorescence
Response Elements
Signal Transduction
Smad Proteins
Zebrafish
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0