A pepsinogen from dog stomach. Comp Biochem Physiol B 1983;75(1):103-7
Date
01/01/1983Pubmed ID
6406133DOI
10.1016/0305-0491(83)90045-7Scopus ID
2-s2.0-0020531183 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
1. A pepsinogen has been purified from dog stomach in 20% yield. 2. At pH values below 4 it spontaneously converts to a pepsin, the first order rate constant at pH 2, 22 degrees C, being 11 min-1. 3. The pepsinogen is stable to denaturation at pH values up to 9, in contrast to the pepsin which is irreversibly denatured above pH 7. 4. Its proteolytic activity against denatured hemoglobin is equal to that of pig pepsin, but in the milk-clotting assay at pH 5.5 its has only 14% the activity.
Author List
Twining SS, Huibregtse K, Glick DMAuthor
Sally S. Twining PhD Assistant Dean, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino AcidsAnimals
Dogs
Enzyme Activation
Gastric Fundus
Gastric Mucosa
Hemoglobins
Hydrogen-Ion Concentration
Pepsin A
Pepsinogens
Protein Denaturation