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The Center for Eukaryotic Structural Genomics. J Struct Funct Genomics 2009 Apr;10(2):165-79

Date

01/09/2009

Pubmed ID

19130299

Pubmed Central ID

PMC2705709

DOI

10.1007/s10969-008-9057-4

Scopus ID

2-s2.0-62949196874   19 Citations

Abstract

The Center for Eukaryotic Structural Genomics (CESG) is a "specialized" or "technology development" center supported by the Protein Structure Initiative (PSI). CESG's mission is to develop improved methods for the high-throughput solution of structures from eukaryotic proteins, with a very strong weighting toward human proteins of biomedical relevance. During the first three years of PSI-2, CESG selected targets representing 601 proteins from Homo sapiens, 33 from mouse, 10 from rat, 139 from Galdieria sulphuraria, 35 from Arabidopsis thaliana, 96 from Cyanidioschyzon merolae, 80 from Plasmodium falciparum, 24 from yeast, and about 25 from other eukaryotes. Notably, 30% of all structures of human proteins solved by the PSI Centers were determined at CESG. Whereas eukaryotic proteins generally are considered to be much more challenging targets than prokaryotic proteins, the technology now in place at CESG yields success rates that are comparable to those of the large production centers that work primarily on prokaryotic proteins. We describe here the technological innovations that underlie CESG's platforms for bioinformatics and laboratory information management, target selection, protein production, and structure determination by X-ray crystallography or NMR spectroscopy.

Author List

Markley JL, Aceti DJ, Bingman CA, Fox BG, Frederick RO, Makino S, Nichols KW, Phillips GN Jr, Primm JG, Sahu SC, Vojtik FC, Volkman BF, Wrobel RL, Zolnai Z

Author

Brian F. Volkman PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Crystallography, X-Ray
Genomics
Humans
Multi-Institutional Systems
Nuclear Magnetic Resonance, Biomolecular
Protein Conformation
Proteins
Proteomics
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