Faculty Collaboration Database

Interleukin 6 promotes vasculogenesis of murine brain microvessel endothelial cells. Cytokine 2000 Jun;12(6):655-65

Date

06/14/2000

Pubmed ID

10843741

DOI

10.1006/cyto.1999.0599

Scopus ID

2-s2.0-0033947016 (requires institutional sign-in at Scopus site)   91 Citations

Abstract

Interleukin 6 (IL-6) is a cytokine that acts on a wide range of tissues influencing cell growth and differentiation. Here we show that IL-6 plays a role in the early vascular development (vasculogenesis) in the central nervous system (CNS). We report that IL-6 induces the proliferation of brain microvascular endothelial cells in vitro. Furthermore, IL-6 significantly accelerates the formation of tube-like structures by these cells in Matrigel basement matrix. Moreover, IL-6 mRNA is expressed in vivo in two physiological conditions in which vascularization in the CNS is important: (1) during normal brain development, (2) during the healing process of a traumatic brain injury. Expression of IL-6 mRNA coincides with the expression of vascular endothelial growth factor (VEGF) mRNA in the developing brain with decreasing expression following birth. However, IL-6 mRNA can be detected in the healing adult murine brain tissue by in situ hybridization coinciding with the period of intense tissue reorganization. The transient upregulation of IL-6 mRNA during normal brain development and at brain injury site and the effect of IL-6 on in vitro vasculogenesis suggest that IL-6 may play a role in normal physiology of vascularization in the CNS.

Author List

Fee D, Grzybicki D, Dobbs M, Ihyer S, Clotfelter J, Macvilay S, Hart MN, Sandor M, Fabry Z

Author

Dominic B. Fee MD Vice Chair, Professor in the Neurology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Basement Membrane
Brain
Cells, Cultured
Cerebrovascular Circulation
Collagen
Drug Combinations
Embryonic and Fetal Development
Endothelial Growth Factors
Endothelium, Vascular
Gene Expression Regulation, Developmental
Interleukin-6
Laminin
Lymphokines
Mice
Mice, Inbred BALB C
Microcirculation
Neovascularization, Physiologic
Proteoglycans
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors