Interleukin 6 promotes vasculogenesis of murine brain microvessel endothelial cells. Cytokine 2000 Jun;12(6):655-65
Date
06/14/2000Pubmed ID
10843741DOI
10.1006/cyto.1999.0599Scopus ID
2-s2.0-0033947016 (requires institutional sign-in at Scopus site) 90 CitationsAbstract
Interleukin 6 (IL-6) is a cytokine that acts on a wide range of tissues influencing cell growth and differentiation. Here we show that IL-6 plays a role in the early vascular development (vasculogenesis) in the central nervous system (CNS). We report that IL-6 induces the proliferation of brain microvascular endothelial cells in vitro. Furthermore, IL-6 significantly accelerates the formation of tube-like structures by these cells in Matrigel basement matrix. Moreover, IL-6 mRNA is expressed in vivo in two physiological conditions in which vascularization in the CNS is important: (1) during normal brain development, (2) during the healing process of a traumatic brain injury. Expression of IL-6 mRNA coincides with the expression of vascular endothelial growth factor (VEGF) mRNA in the developing brain with decreasing expression following birth. However, IL-6 mRNA can be detected in the healing adult murine brain tissue by in situ hybridization coinciding with the period of intense tissue reorganization. The transient upregulation of IL-6 mRNA during normal brain development and at brain injury site and the effect of IL-6 on in vitro vasculogenesis suggest that IL-6 may play a role in normal physiology of vascularization in the CNS.
Author List
Fee D, Grzybicki D, Dobbs M, Ihyer S, Clotfelter J, Macvilay S, Hart MN, Sandor M, Fabry ZAuthor
Dominic B. Fee MD Vice Chair, Professor in the Neurology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AgingAnimals
Basement Membrane
Brain
Cells, Cultured
Cerebrovascular Circulation
Collagen
Drug Combinations
Embryonic and Fetal Development
Endothelial Growth Factors
Endothelium, Vascular
Gene Expression Regulation, Developmental
Interleukin-6
Laminin
Lymphokines
Mice
Mice, Inbred BALB C
Microcirculation
Neovascularization, Physiologic
Proteoglycans
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors