Characterisation of expression patterns and functional role of Cactin in early zebrafish development. Gene Expr Patterns 2010 Jun;10(4-5):199-206
Date
03/30/2010Pubmed ID
20348034DOI
10.1016/j.gep.2010.03.003Scopus ID
2-s2.0-77956632707 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
The immune system of teleost zebrafish (Danio rerio) shows high similarity to mammalian counterparts sharing many innate immune components including Toll-Like Receptors (TLRs), cytokines, chemokines and complement molecules. As in mammals, zebrafish also contains the transcription factor NF-kappaB that plays dualist roles in innate immunity and early development. Indeed NF-kappaB members are expressed in different temporal patterns during the early stages of zebrafish embryogenesis indicating that each molecule is involved in specific developmental events. In the present study we employ zebrafish as a model to characterise the expression pattern and role of a novel NF-kappaB regulator, termed Cactin, in early development. Cactin was first characterised in Drosophila as a new member of the Rel pathway that could affect the generation of dorsal-ventral polarity. To explore the potential developmental role of Cactin in zebrafish, we initially investigated its expression pattern and functional role during early embryonic developmental stages. We detect Cactin expression at all stages of early development and knockdown of Cactin by specific morpholino antisense oligonucleotides causes developmental abnormalities manifested by an overall dysmorphic cellular organisation. These results indicate that Cactin has been highly conserved during evolution and plays a key role in early embryonic development.
Author List
Atzei P, Yang F, Collery R, Kennedy BN, Moynagh PNAuthor
Ross F. Collery PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBase Sequence
Carrier Proteins
DNA Primers
Drosophila Proteins
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Humans
Polymerase Chain Reaction
Zebrafish