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[Ion channels in rat pancreatic beta cells]. Sheng Li Xue Bao 2000 Apr;52(2):98-102

Date

04/19/2002

Pubmed ID

11961576

Scopus ID

2-s2.0-0347741450 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

Using perforated and cell-attached patch clamp techniques, the characteristics of ATP sensitive K(+) channels (K(ATP)), delayed rectifier K(+) channels (K(DR)), Ca(2+) and Na+ channels on single rat pancreatic beta cell membranes were studied. The results showed that (1) the efflux and influx conductance of K(ATP) channels was about 31 and 65 pS respectively, and the reversal potential of K(ATP) was about 60 mV; (2) K(DR) was activated completely after a latency of 20 ms, and K(DR) was about 1/3 of K(ATP); (3) whole cell Ca(2+) current reached a peak (40 60 pA) at 0 mV; L-type Ca(2+) channel was the main Ca(2+) channel in beta cells, but other types of high voltage activated Ca(2+) channels existed as well; and 4) whole cell Na(+) current reached a peak (200 400 pA) at 10 mV; but the expression level of Na(+) channel in beta cells varied among the cells. About half of the beta cells virtually had no Na(+) currents.

Author List

Zeng XH, Lou XL, Qu AL, Wu HX, Zhou Z

Author

Xuelin Lou PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Calcium Channels
Cells, Cultured
Islets of Langerhans
Patch-Clamp Techniques
Potassium Channels
Rats
Sodium Channels
Sodium-Potassium-Exchanging ATPase