Selective killing of leukemic cells by merocyanine 540-mediated photosensitization. Proc Natl Acad Sci U S A 1984 Dec;81(23):7584-7
Date
12/01/1984Pubmed ID
6594700Pubmed Central ID
PMC392191DOI
10.1073/pnas.81.23.7584Scopus ID
2-s2.0-0344041416 (requires institutional sign-in at Scopus site) 148 CitationsAbstract
In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.
Author List
Sieber F, Spivak JL, Sutcliffe AMAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBone Marrow
Cell Survival
Colony-Forming Units Assay
Hematopoietic Stem Cells
Leukemia L1210
Light
Mice
Pyrimidinones
Radiation-Sensitizing Agents