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Selective killing of leukemic cells by merocyanine 540-mediated photosensitization. Proc Natl Acad Sci U S A 1984 Dec;81(23):7584-7

Date

12/01/1984

Pubmed ID

6594700

Pubmed Central ID

PMC392191

DOI

10.1073/pnas.81.23.7584

Scopus ID

2-s2.0-0344041416 (requires institutional sign-in at Scopus site)   148 Citations

Abstract

In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

Author List

Sieber F, Spivak JL, Sutcliffe AM

Author

Fritz Sieber PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Bone Marrow
Cell Survival
Colony-Forming Units Assay
Hematopoietic Stem Cells
Leukemia L1210
Light
Mice
Pyrimidinones
Radiation-Sensitizing Agents