SEPT5_v2 is a parkin-binding protein. Brain Res Mol Brain Res 2003 Oct 07;117(2):179-89
Date
10/16/2003Pubmed ID
14559152DOI
10.1016/s0169-328x(03)00318-8Scopus ID
2-s2.0-12144290241 (requires institutional sign-in at Scopus site) 110 CitationsAbstract
Mutations in parkin are associated with various inherited forms of Parkinson's disease (PD). Parkin is a ubiquitin ligase enzyme that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins destined for proteasomal degradation. The substrates of parkin-mediated ubiquitination have yet to be completely identified. Using a yeast two-hybrid screen, we isolated the septin, human SEPT5_v2 (also known as cell division control-related protein 2), as a putative parkin-binding protein. SEPT5_v2 is highly homologous to another septin, SEPT5, which was recently identified as a target for parkin-mediated ubiquitination. SEPT5_v2 binds to parkin at the amino terminus and in the ring finger domains. Several lines of evidence have validated the putative link between parkin and SEPT5_v2. Parkin co-precipitates with SEPT5_v2 from human substantia nigra lysates. Parkin ubiquitinates SEPT5_v2 in vitro, and both SEPT5_v1 and SEPT5_v2 accumulate in brains of patients with ARJP, suggesting that parkin is essential for the normal metabolism of these proteins. These findings suggest that an important relationship exists between parkin and septins.
Author List
Choi P, Snyder H, Petrucelli L, Theisler C, Chong M, Zhang Y, Lim K, Chung KK, Kehoe K, D'Adamio L, Lee JM, Cochran E, Bowser R, Dawson TM, Wolozin BMESH terms used to index this publication - Major topics in bold
AgedAged, 80 and over
Brain
Case-Control Studies
Cells, Cultured
Female
Humans
Immunoblotting
Immunohistochemistry
In Vitro Techniques
Kidney
Male
Middle Aged
Nerve Tissue Proteins
Neuroblastoma
Parkinson Disease
Plasmids
Precipitin Tests
Protein Binding
Proto-Oncogene Proteins
Sequence Homology, Nucleic Acid
Transcription Factor RelB
Transcription Factors
Transfection
Two-Hybrid System Techniques
Ubiquitin
Ubiquitin-Protein Ligases