Oxidized phosphatidylserine-CD36 interactions play an essential role in macrophage-dependent phagocytosis of apoptotic cells. J Exp Med 2006 Nov 27;203(12):2613-25
Date
11/15/2006Pubmed ID
17101731Pubmed Central ID
PMC2118161DOI
10.1084/jem.20060370Scopus ID
2-s2.0-33751519372 (requires institutional sign-in at Scopus site) 351 CitationsAbstract
The phagocytosis of apoptotic cells within an organism is a critical terminal physiological process in programmed cell death. Evidence suggests that apoptotic cell engulfment and removal by macrophages is facilitated by phosphatidylserine (PS) displayed at the exofacial surface of the plasma membrane; however, neither the macrophage receptors responsible for PS recognition, nor characterization of the PS molecular species potentially involved, have been clearly defined. We show that the class B scavenger receptor CD36 plays an essential role in macrophage clearance of apoptotic cells in vivo. Further, macrophage recognition of apoptotic cells via CD36 is shown to occur via interactions with membrane-associated oxidized PS (oxPS) and, to a lesser extent, oxidized phosphatidylcholine, but not nonoxidized PS molecular species. Mass spectrometry analyses of oxPS species identify structures of candidate ligands for CD36 in apoptotic membranes that may facilitate macrophage recognition. Collectively, these results identify oxPS-CD36 interactions on macrophages as potential participants in a broad range of physiologic processes where macrophage-mediated engulfment of apoptotic cells is involved.
Author List
Greenberg ME, Sun M, Zhang R, Febbraio M, Silverstein R, Hazen SLAuthor
Roy L. Silverstein MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsApoptosis
CD36 Antigens
COS Cells
Cells, Cultured
HL-60 Cells
Humans
Jurkat Cells
K562 Cells
Macrophages
Mice
Mice, Inbred C57BL
Mice, Knockout
Oxidation-Reduction
Phagocytosis
Phosphatidylserines
